WP1 – PROJECT MANAGEMENT

Objectives

  • guarantee of an efficient project administration and coordination
  • scientific and technical support for the realization of all activities
  • communication with all team members and the Croatian Science Foundation

Description of work:

A.1.1 Kick-off meeting

A.1.2 Meetings organization

  • WP leaders meetings
  • individual WP groups meetings
  • meetings of all team members

A.1.3 Data collection and final periodic report preparation

Deliverables & Milestones:

D.1.1 Report of Kick-off meeting

D.1.2 Reports of the official meetings

D.1.3 Periodical reports and final report (financial and technical)

M.1.1 Kick-off meeting

M.1.2 First year final report

WP2 – SCAFFOLDS FABRICATION

Objectives

  • design of scaffolds tailored topography and dual porosity for the improvement of cells adhesion and in-depth penetration by electrospinning on custom designed patterned metal collectors and combined electrospinning (solution and melt)
  • design of scaffolds with antibacterial properties, by the addition of different shaped and sized TiO2 particles (nano, mico and nanotbes) into the polymer solution or melt
  • design of scaffolds with controlled pace release function of cells growth factors
  • combo electrospun scaffolds with multifunctional properties and optimal architecture

Description of work:

A.2.1 Purchase of a melt electrospinning device

A.2.2 Fabrication of innovated metal collectors

  • design of metal flat plates with different sized pores and pores distribution (associated with cells type and size)

A.2.3 Combined electrospinning

  • dual porosity will be realized by combination of nano solution electrospun fibers and micro melt electrospun fibers
  • the total dual structure will be realized by alteration of the nano and micro fibrous layers

A.2.4 Optimal materials composition and processing parameters selection

  • preparation of the polymers solutions and melts from both synthetic and natural polymers
  • preparation of the polymers solutions and melts from both synthetic and natural polymers in a blend with antibacterial TiO2 (nano, micro, nanotubes) particles
  • preparation of the polymers solutions and melts from both synthetic and natural polymers in a blend with cells growth factors

Deliverables & Milestones:

D.2.1 Scaffolds with newly developed patterned topography

D.2.2 Scaffolds with newly developed inner structure

D.1.3 Scaffolds with antibacterial properties and scaffolds with biological compound delivery function

M.2.1 Combination of scaffolds desired architecture and multifunctionality

WP3 – EVALUATION OF SCAFFOLDS STRUCTURE AND FUNCIONALITY

Objectives

  • confirmation of scaffolds desired physical and chemical characteristics
  • optimal nano/micro fibrous scaffolds structure selection
  • optimal wetting ability, antibacterial property and biological compound controlled release property selection
  • systematic study on the optimal selected samples including influential parameters (processing and materials aspect) on scaffolds desired design and functionality

Description of work

A.3.1 Characterization of the scaffolds physical structure

  • evaluation of scaffolds morphology (fiber diameter, fiber alignment/anisotropy, topography), using SEM and TEM
  • evaluation of scaffolds porosity (pore size, pore volume, surface area, interconnectivity, tortuosity), using porosimeter

A.3.2 Thermal stability and crystallinity testing, using TGA and DSC
A.3.3 Mechanical properties testing (static and dynamic as a function of temperature), using Tensile tester and DMA

  • tests performance under room or physiological condition

A.3.4 Chemical composition analysis, using ATR-FTIR
A.3.5 Surface wetting ability, using Goniometer

  • water contact angle measurement

A.3.6 Degradation tests on scaffolds prior cell culture

  • measurement of mass loss after enzymatic hydrolysis

A.3.7 Evaluation of the scaffolds release profile in PBS as a function of time, using UV–vis spectroscopy

Deliverables & Milestones:

D.3.1 Preliminary report on scaffolds encapsulation ability in regard to antibacterial and biological compounds incorporation

D.3.1 Systematic study on scaffolds degradability with time, based on biodegradable polymers and silk fibroin

D.3.3 Systematic study on scaffolds controlled pace release of the biological compound

M.3.1 Fulfilment of desired requirements

WP4 – VALIDATION OF THE SCAFFOLDS ARCHITECTURE AND FUNCIONALITY IN VITRO

Objectives

  • enhancement of cells adhesion and surface spreading, cells infiltration and homogenous colonization in relation to scaffolds topography, wettability and inner fibrous architecture
  • enhancement of cells viability and differentiation in relation to cytotoxicity and carcinogenicity tests
  • monitoring of scaffolds cellular stimulating property with adjustable pace release of the biological (cells growth factors) compounds in relation with new tissue growth
  • monitoring of the scaffolds degradability with time in relation with new tissue growth

Description of work

A.4.1 Cells seeding

  • skin and ocular cells culture

A.4.2 Observation of cells surface adhesion and spreading

  • SEM analysis

A.4.3 Testing of cells viability and differentiation

  • immunological, microbiological, biochemicals and enzyme activity measurements on cultured cells by fluorometry

A.4.4 Antibacterial tests

  • contact kill test (ISO 22196)

A.4.5 Cytotoxicity and carcinogenicity tests

  • performing cytotoxicity assays to measure the release of LDH (lactate dehydrogenase) from damaged cells
  • performing Soft Agar Assay for colony formation to detect malignant transformation of cells

Deliverables & Milestones:

D.4.1 Confirmation of the newly developed scaffolds multifunctionality in vitro
D.4.2 Periodical reports after scaffolds each feature validation
M.4.1 Fulfilment of desired requirements

WP5 – DISSEMINATION AND EXPLOITATION

Objectives

  • dissemination and exploitation of project resulted knowledge
  • publication of the newly developed knowledge in international peer-reviewed scientific journals
  • organization of workshops and presentation of the knowledge in international conferences
  • organization of invited lectures in the project study field

Description of work

A.5.1 Preparation of the project web page

A.5.2 Knowledge transfer

  • knowledge transfer to young researchers
  • knowledge transfer to target groups (e.g. knowledge transfer to Tissue Bank, University Hospital Sisters of Charity, Clinic for Traumatology and Specialty Eye Hospital “Svjetlost”

A.5.3 Conferences participation

A.5.4 Publications

  • periodical publication of journal papers

A.5.5 Scientific training

A.5.6 Popularisation of science

  • media promotion of the project

Deliverables & Milestones:

D.5.1 Public availability of the project progress and dissemination of the results

D.5.2 Final and graduate thesis works

D.5.3 Papers in conference proceedings

D.5.4 Published papers in journals

D.5.5 Individual scientific progress and potential collaborations

D.5.6 Press-release, TV and radio

M.5.1 Web page setting